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Photosynthetic sugar can drive spatial waves of clock gene expression by altering periods locally.

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posted on 2019-08-15, 17:29 authored by Mark Greenwood, Mirela Domijan, Peter D. Gould, Anthony J. W. Hall, James C. W. Locke

(A) Period estimates of GI::LUC for different organs during the inhibition of photosynthesis by DCMU. ***p < 0.001, by two-tailed t test, Welch correction. (B) Times of peaks of of GI::LUC expression in different organs during the inhibition of photosynthesis by DCMU. Color legend is as in A. Plots represent the 25th percentile, median, and the 75th percentile for the peak times of organs scored as rhythmic. (C) Representative phase plot of GI::LUC expression across longitudinal sections of the hypocotyl and root of a single seedling during the inhibition of photosynthesis by DCMU. (D) Schematic representing the experimental design. Seedlings are cut at the hypocotyl junction and the excised root laid across two adjacent agar pads, one containing sugar-supplemented media and the other not, so that only the top part of the root is in contact with sugar. Roots are then imaged under DD. (E) Period estimates of GI::LUC for the top and middle regions of the root during the partial contact of the root with sucrose or mannitol, under DD. ***p < 0.001, by two-tailed t test, Welch correction. (F) Times of peaks of GI::LUC expression for the top and middle regions of the root during the partial contact of the root with exogenous sucrose or mannitol, under DD. Plots represent the 25th percentile, median, and the 75th percentile for the peak times of organs scored as rhythmic. (G, H) Representative phase plot of GI::LUC expression across longitudinal sections of the hypocotyl and root of a single seedling during the partial contact of the root with exogenous sucrose (G) or mannitol (H), under DD. Schematic shows the approximate position of the root on the agar pads. Color bars are as in C. For DCMU, N = 3; exogenous sucrose, N = 3; exogenous mannitol, N = 2. For all, n = 8–30. N represents the number of independent experiments and n the total number of organs tracked. See S1 and S2 Files for exact n, test statistics, and percentage rhythmicity in each organ. Box plots indicate the median and upper and lower quartiles, and whiskers the 9th and 91st percentiles of organs scored as rhythmic. Underlying data are available from https://gitlab.com/slcu/teamJL/greenwood_etal_2019. DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea; DD, constant darkness; GI, GIGANTEA; LL, constant light; LUC, LUCIFERASE; RT, root tip.

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