Fig 2.tif (2.45 MB)
Phenotype of DKO mice.
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posted on 2017-12-29, 18:34 authored by Miki Shimbo, Riku Suzuki, Sayaka Fuseya, Takashi Sato, Katsue Kiyohara, Kozue Hagiwara, Risa Okada, Hiromasa Wakui, Yuki Tsunakawa, Hideto Watanabe, Koji Kimata, Hisashi Narimatsu, Takashi Kudo, Satoru Takahashi(A) Representative photograph of t2 KO pups and cyanotic DKO littermates at E18.5. Scale bar: 1 cm. (B) Body weight of t2 KO (n = 68) and DKO (n = 51) embryos at E18.5. *P < 0.05. (C) Lung sections from t2 KO embryos and DKO littermates were stained with HE. Scale bar: 100 μm. (D-H) Skeletal whole body (D), upper limb (E), lower limb (F), lumber spine (G), and cranial bone (H) preparation. (I) Humeral and tibial bone length at E18.5 of t2 KO (n = 5) and DKO (n = 10) mice. **P < 0.01.
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WTQuantitative RT-PCR analysist 2t 1CS synthesiscartilage-specific t 1t 1 KO miceCol 2a 1chondrocyteendochondral ossificationECMCS contentPtch 1 transcriptst 2 KO micechondroitin sulfate NGAG expression levelst 1 KO cartilage-2 Chondroitin sulfateCol 2-DKO miceDKO cartilaget 1 KOE 18.5 cartilageCol 2-DKO knee cartilage
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