pone.0230915.g007.tif (930.54 kB)
PCR amplifications under stringent annealing temperatures.
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posted on 2020-03-27, 20:05 authored by Jenq-Kuen Huang, Kadidia Samassekou, Hekmat B. Alhmadi, David R. VanDerway, Joshua D. Diaz, Jacob A. Seiver, Shawn W. McClenahan, Scott M. Holt, Lisa Wen(A) amplification of the 1.1 kb fragments using primer pair 13+17. (B) amplification of the 0.8 kb fragments using primer pair 9+18. (C) amplification of the 1.2 kb fragments using primer pair 9+20. The templates used were genomic DNA isolated from clone 1-p-11, knockout mutants 1-3-17 and/or 2-3-52. Annealing temperatures used are indicated on top of each lane.
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Nocardia cholesterolicum NRRL 5767. Existencealcohol dehydrogenase geneknockout mutants offer improvementsoleate hydratase isozymesNocardia cholesterolicum NRRL 5767Nocardia cholesterolicum NRRL 5767DNAGolden Gate Assemblyalcohol dehydrogenase activityNocardia cholesterolicum NRRL 5767 strainalcohol dehydrogenase knockout mutants10- hydroxystearic acidoleic acid10- ketostearic acidCRISPRalcohol dehydrogenase
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