Fig 2.tif (843.14 kB)

Overlap of OTUs generated using Uclust between/among technical replicates at different sequencing depth.

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posted on 2017-04-28, 17:28 authored by Chongqing Wen, Liyou Wu, Yujia Qin, Joy D. Van Nostrand, Daliang Ning, Bo Sun, Kai Xue, Feifei Liu, Ye Deng, Yuting Liang, Jizhong Zhou

At a sequencing depth of about 30,000 reads, overlap of both two and three replicates was approaching a plateau when singletons were not removed.

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treatment effects amplicon sequencing 16 S rRNA gene amplicons V 4 region 16 S rRNA gene UMI sequence abundance MiSeq 18 soil samples gene amplicon sequencing PCR replicate reproducibility Detrended correspondence analysis OTU sequence abundance OTU overlap

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