Overexpressing <i>lincRNA-IBIN</i> improves the survival of <i>Drosophila</i> adults from an infection and the expression of selected target genes.

<p><b>A)</b><i>UAS-lincRNA-IBIN</i> (<i>CR44404</i>) overexpression with the <i>C564-GAL4</i> driver (<i>C564>lincRNA-IBIN</i><sup><i>1</i></sup> and <i>C564>lincRNA-IBIN</i><sup><i>7</i></sup>) significantly increases the expression of <i>lincRNA-IBIN</i> in uninfected flies measured with qPCR, as does an infection with <i>M</i>. <i>luteus</i>. <b>B)</b> <i>lincRNA-IBIN</i> overexpression (<i>C564>lincRNA-IBIN</i><sup><i>7</i></sup>) improves the survival of the flies after an infection with <i>M</i>. <i>luteus</i> + <i>E</i>. <i>faecalis</i>. <b>C-D)</b> <i>lincRNA-IBIN</i> overexpression increases the expression of two Toll pathway target genes <i>IM1</i> <b>(C)</b> <i>and Drosomycin</i> <b>(D)</b> after a <i>M</i>. <i>luteus</i> infection. <i>Myd88</i> knockdown flies were used as a negative control and <i>cactus</i> knockdown flies as a positive control. In <b>A, C</b> and <b>D</b>, for fold-induction values, expression values in uninfected <i>w</i>, <i>lincRNA-IBIN</i><sup><i>7</i></sup> samples were set to 1. <b>E)</b> <i>E</i>. <i>cloacae</i>-induced endogenous <i>lincRNA-IBIN</i> expression is lost upon <i>Relish</i> RNAi. <i>lincRNA-IBIN</i> overexpression is shown in the Relish RNAi background. <b>F)</b> Upon a <i>E</i>. <i>cloacae</i> infection, <i>lincRNA-IBIN</i> overexpression gives a statistically significant survival advantage to flies with a <i>Relish</i> RNAi background. <b>G)</b> <i>Attacin C</i> (<i>AttC</i>) is not produced in <i>Relish</i> RNAi flies, but with IBIN overexpression a very small amount of <i>AttC</i> is induced. H) <i>Diptericin A</i> (<i>DptA</i>) is not produced with or without <i>lincRNA-IBIN</i> overexpression in flies with a <i>Relish</i> RNAi background upon a <i>E</i>. <i>cloacae</i> infection. In <b>E, G</b> and <b>H</b>, for fold-induction values, expression values in uninfected <i>w</i>, <i>Rel</i><sup><i>IR</i></sup> samples were set to 1.</p>