Overexpressing lincRNA-IBIN improves the survival of Drosophila adults from an infection and the expression of selected target genes.
A)UAS-lincRNA-IBIN (CR44404) overexpression with the C564-GAL4 driver (C564>lincRNA-IBIN1 and C564>lincRNA-IBIN7) significantly increases the expression of lincRNA-IBIN in uninfected flies measured with qPCR, as does an infection with M. luteus. B) lincRNA-IBIN overexpression (C564>lincRNA-IBIN7) improves the survival of the flies after an infection with M. luteus + E. faecalis. C-D) lincRNA-IBIN overexpression increases the expression of two Toll pathway target genes IM1 (C) and Drosomycin (D) after a M. luteus infection. MyD88 knockdown flies were used as a negative control and cactus knockdown flies as a positive control. In A, C and D, for fold-induction values, expression values in uninfected w, lincRNA-IBIN7 samples were set to 1. E) E. cloacae-induced endogenous lincRNA-IBIN expression is lost upon Relish RNAi. lincRNA-IBIN overexpression is shown in the Relish RNAi background. F) Upon a E. cloacae infection, lincRNA-IBIN overexpression gives a statistically significant survival advantage to flies with a Relish RNAi background. G) Attacin C (AttC) is not produced in Relish RNAi flies, but with IBIN overexpression a very small amount of AttC is induced. H) Diptericin A (DptA) is not produced with or without lincRNA-IBIN overexpression in flies with a Relish RNAi background upon a E. cloacae infection. In E, G and H, for fold-induction values, expression values in uninfected w, RelIR samples were set to 1.