Osteoclasts generated with S100A12 exhibit increased bone resorption.

Osteoclasts were generated on a bone resorption assay plate with or without S100A12 (1,000 ng/ml) for 16 days. The resorption lacunae were examined by microscopy. (A) The percentage of the resorbed area (i.e., bright areas in (B)) was quantified and expressed as the mean and SD of 3 independent experiments. * = p < 0.05; ** = p < 0.01. (B) Representative images of resorption pit formation (original magnification × 200).