Notch signaling was induced in the Pea3 mutant lacrimal gland.

(A-B) GSEA analysis and Volcano plot data revealed that Notch pathway genes were enriched in Pea3 mutants. (C) The Pea3 knockout resulted in loss of the Notch regulator Lfng, induction of ligand Jag1 and ectopic expression of the effector, Notch-ICD, in the lacrimal gland bud (dotted lines). Scale bar, 50μm.