Fig 7.tif (339.84 kB)
Nonenzymatic glycosylation of human serum albumin and its effect on antibodies profile in patients with diabetes mellitus - Fig 7
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posted on 2017-05-17, 17:37 authored by Alok Raghav, Jamal Ahmad, Khursheed Alam(a) Inhibition of binding of affinity purified anti-HSA IgG and pre-imune IgG to native HSA. Microtitre plates were coated with native HSA (10 μg/mL). (b) Inhibition of binding of affinity purified anti-glucose-HSA IgG and pre-imune IgG to glucose modified HSA. Microtitre plates were coated with native HSA (10 μg/mL).
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CKDNonenzymatic glycosylation1737 cm -1. LCMS chromatogramHSA manifestsmgcarbonyl groupglucoseCMLdiabetes mellitus type 2diabetes mellitustype 2glycated-HSAUVGDMantibodyAmide II peakneo-epitopes generationcomplicationpeak appearancecarboxymethyl lysineModified samplesλ 280 nm2DManincreasein fluorescence intensitybinding enzyme-linked immunosorbent assaykidney diseaseauto-antibodies formationFTIR resultsConclusions Non-enzymatic glycosylationanantigen concentrationdiabetes mellitus Background Albumin glycationResults Glycated-HSANeo-epitopesagainst In-vitroLiquid chromatography mass spectrometryantibodies profilebinding ELISAamarked increase1DMglycation end products40 day periodMethods Human Serum Albuminserum albumin
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