Neutrophil accumulation is reduced with A33 treatment at 24 hrs after TBI.

Representative CD45/CD11b and CD45/RP-1 scatter plots from the ipsilateral parietal cortex of (A) vehicle-treated and (B) A33-treated animals at 3 hrs post-injury. CD45/RP-1 scatter plots were gated on total CD11b⁺ cells. (C) Quantification of total CD11b⁺ cells, microglia, infiltrating CD11b⁺ cells and neutrophils in vehicle-treated and A33-treated TBI animals at 3 hrs post-injury. (D) Percentage of infiltrating CD11b⁺ cells that are neutrophils (CD45^high, CD11b⁺, RP-1⁺). Representative CD45/CD11b and CD45/RP-1 scatter plots at 24 hrs post-injury in (E) vehicle-treated and (F) A33-treated animals. (G) Quantification of total CD11b⁺ cells, microglia, infiltrating CD11b⁺ cells and neutrophils. (H) Percentage of infiltrating CD11b⁺ cells that are neutrophils at 24 hrs after TBI. Mean ± SEM, n = 10/group (3 hrs), n = 5/group (24 hrs), **p<0.01 vs. TBI+Vehicle, Student’s unpaired t-test. (RP-1 = rat neutrophil marker).