Fig 3.tif (8.35 MB)

NRTN gene transfection increases TH(+) fibers in the striatum of rats with chronic 6-OHDA lesion.

figure

posted on 2017-11-27, 18:31 authored by David Reyes-Corona, Nallely Vázquez-Hernández, Lourdes Escobedo, Carlos E. Orozco-Barrios, Jose Ayala-Davila, Mario Gil Moreno, Miriam E. Amaro-Lara, Yazmin M. Flores-Martinez, Armando J. Espadas-Alvarez, Manuel A. Fernandez-Parrilla, Juan A. Gonzalez-Barrios, ME Gutierrez-Castillo, Ignacio González-Burgos, Daniel Martinez-Fong

A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas [54]. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 1 mm is for slices at different levels and of 100 μm for details. B. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.