Mutants of the candidate gene for qTA3 showing a larger tiller angle.
(a) A representation of pairwise r2 values (a measure of LD) all polymorphic sites between Os03g51660 and Os03g51670, where the darkness of the color of each box corresponds to the r2 value according to the legend. The line in red color represents lead SNP. (b) Schematic diagram of Os03g51660 and Os03g51670 structures with the T-DNA insertion. The black squares represent exons, and the black lines represent introns. The red line means the position of the lead SNP of qTA3; the triangles indicate the positions of T-DNA insertion in three mutants; L and R, left and right genomic primers; N, vector primer. (c) (d)Genotyping of the mutants tac3-1D and tac3-2D. W, wild type; H, heterozygote; M, homozygote. (e) qRT-PCR expression analysis of Os03g51660 in wild type (ZH11) and tac3-1D mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥ 9, *** significant difference between mutants and wild type at p<0.001. (f) qRT-PCR expression analysis of Os03g51670 in ZH11 and tac3-1D mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥4 plants, *** significant difference between mutants and wild type at p<0.001. (g)-(i) The tiller angle of ZH11, heterozygote (tac3-1D H) and homozygote (tac3-1D M) mutant at tillering and heading stages; ** and *** significant difference between mutants and wild type at p<0.01 and p<0.001, respectively. (j) qRT-PCR expression analysis of Os03g51660 in wild type (DJ) and tac3-2D mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥4 plants, *** significant difference between mutants and wild type at p<0.001. (k) qRT-PCR expression analysis of Os03g51670 in DJ and tac3-2D mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥4 plants. (l)-(n) The tiller angle of DJ, heterozygote (tac3-2D H) and homozygote (tac3-2D M) mutant at tillering and heading stages; the total plant number (n) = 62 plants, ** significant difference between mutants and wild type at p<0.01.
