Morphological changes in A549 cells stained with acridine orange (AO) and ethidium bromide (EB).
Cells were untreated (A) or treated for 3 h with 1 μM staurosporine (B), 10 μM β-hederin (C), 5 μM Bi-L-RhamBet (D), 10 μM Bi-L-RhamBet (E), and 20 μM Bi-L-RhamBet (F). A549 cells were stained with AO/EB for 5 min and then visualized using fluorescence microscopy. Arrows indicate apoptotic blebbing. This analysis is representative of three independent experiments.