Molecular identification and genetic complementation of the HPS5 gene.
(A), Diagrams showing the positions of the mutations in the ERS1 gene and ERS1 protein. Top: the structure of the ERS1 gene. Black box: exons; grey box: 5’ and 3’ UTRs; thin line: introns; thick line: flanking sequences surrounding the ERS1 gene. Bottom: the structure of the ERS1 protein showing the signature domains. (B), Morphology of 7-day-old seedlings and root tips of the WT, hps5, and complementation lines grown on P+ and P- media. (C), Quantitative analyses of root hair density and root hair length of various lines. Values represent means ± SD of three replicates. Asterisks indicate a significant difference from the WT (t-test, P < 0.05). For each measurement, 15–20 seedlings were used.