Fig 4.TIF (245.71 kB)
Michaelis−Menten kinetics of dansylcadaverine and N,N-dimethylcasein conjugation by human FXIIIa in the presence of NSGM 13.
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posted on 2016-07-28, 12:29 authored by Rami A. Al-Horani, Rajesh Karuturi, Michael Lee, Daniel K. Afosah, Umesh R. DesaiThe initial rate of conjugation at (A) various dansylcadaverine concentrations (0–750 μM) and fixed dimethylcasein concentration (5 mg/mL) or (B) various dimethylcasein concentrations (0–5 mg/mL) and fixed dansylcadaverine concentration (250 μM) was measured spectrofluorometrically in pH 8.0 buffer at 37°C. Solid lines represent nonlinear regressional fits to the data by the standard Michaelis− Menten Eq 4 to yield KM and VMAX. See details in Materials and Methods.
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NSGMallosteric processFluorescence studies22 variably sulfated GAGsnon-saccharide GAG mimeticsorthosteric inhibitionnovel FXIIIa inhibitorscysteine protease papainallosteric FXIIIa inhibitorsallosteric mechanismsulfated non-saccharide agentsNon-Saccharide Glycosaminoglycan MimeticsAllosteric InhibitionExhibit Promising Inhibition Profile Factor XIIIaacid residuesFXIIIa-mediated polymerizationsulfated glycosaminoglycansFactor XIIIabinding affinityinhibition potencyallosteric inhibitionGAG-binding proteins factor Xaundecasulfated quercetin analogdesign FXIIIa inhibitorscoagulation process
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