pone.0170318.g004.tif (1.08 MB)
Metformin inhibits grade 1 endometrial cancer cell proliferation, survival, migration, and induces apoptosis.
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posted on 2017-01-23, 17:39 authored by John J. Wallbillich, Srirama Josyula, Uksha Saini, Roman A. Zingarelli, Kalpana Deepa Priya Dorayappan, Maria K. Riley, Ross A. Wanner, David E. Cohn, Karuppaiyah SelvendiranA. Sulforhodamine B (SRB) assay measured Ishikawa cell proliferation with increasing concentrations of metformin after 24h or 48h, in high-glucose media. B. Proportion of Ishikawa cells surviving (compared to control) after treatment with 10 mM or 20 mM of metformin. C. Cell migration assay, with Ishikawa cells subjected to control, 10 mM, or 20 mM metformin for 24h. Control (0h) is also shown. D. Quantification of % wound closure; the 10 mM and 20 mM treatment groups were each significantly different than control (p<0.05; noted with asterisk [*]). E. Western blot of apoptosis-related protein expression in Ishikawa cells after treatment with control, 10 mM, or 20 mM for 48h.
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STAT 3 overexpression plasmidtarget proteinsHigh Glucose-Mediated STAT 3 ActivationIshikawa cells transfectedEndometrial Cancer Objectives STAT 3xenograft tumor modelWestern blottype 1 endometrial cancerapoptotic protein expressionPCRglucose concentrations influence STAT 3 expressionSTAT 3 overexpressionSTAT 3pSTAT 3 ser 727.cell proliferationmetformin treatmentIshikawa cellsSTAT 3 expression
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