Meiotic initiation and progression in XX, XO and XY PGCLC/oocytes.

(A) Meiotic initiation in XX, XO and XY PGCLC/oocytes. Images show the immunofluorescence analysis of SYCP3 (green) and NANOG (red) in PGCLCs/oocytes at agg-7. Scale bars, 50 μm. (B) Time-course of meiotic entry. The graph shows the average percentage and SD of SYCP3- or NANOG-positive cells at the days indicated. The data were compiled from two independent experiments. (C) EdU incorporation into NANOG-positive cells. Images show the immunofluorescence analysis of BV/SC (green), NANOG (red), EdU (Blue) and their merged images in rOvaries. The number below the images indicates NANOG-positive cells per EdU-positive cells. Scale bars, 50 μm. (D) The percentage of EdU-positive cells per BV- and/or SC-positive cells. The graph indicates the average percentage and SD of EdU-positive cells per BV- and/or SC-positive cells. The data were compiled from two independent experiments. (E) Schematic diagram of the reconstitution experiment using PGCs derived in vivo. XX or XY PGCs of E10.5 embryos were reaggregated with gonadal somatic cells of E12.5 female embryos. (F) The percentage of cells entering meiosis. The graph shows the average percentage and SD of SYCP3- or NANOG-positive cells at the days indicated. The data was compiled from four independent experiments. P values were calculated by t-test. ***P<0.001. (G) NANOG-positive XY germ cells in the rOvary. Images show the immunofluorescence analysis of SYCP3 (green), NANOG (red), TRA98 (a marker of germ cells: white) and their merged images. The dashed lines indicate representative NANOG-positive germ cells. Scale bars, 50 μm.