Measured versus actual viability of cercariae as determined by the dyes used in this study.

Samples contained 100 ± 4 cercariae (ranging from 0–100% viable) and were stained with FDA-PI and FDA-H. Data points and error bars show the mean and SEM of nine wells (experiments run in triplicate and repeated three times). “Actual” viabilities are the known proportions of live and dead cercariae in each well, and “measured” viabilities are those quantified with the fluorescence data.