LysC treatment (37 ˚C, 10 min) of DDM-MraY and SMA-MraY.
The influence of 0.1% DDM on the stability of SMA-MraY in the presence of LysC is shown after 10, 40 and 120 min incubation with the detergent at a final concentration of 0.1%. The lanes with the untreated protein are annotated with (*). M indicates the protein marker and the sizes (in kDa) are shown on the left side of the ladder. Intact MraY displays a molecular weight of about 31 kDa and is indicated by a black arrow.