Lung infection experiments in K. pneumoniae ATCC 13883 and 10.85 as well as their isogenic porin mutants ΔOpmK35/OmpK36GD.
Survival curves after intranasal infection are represented in panels A1 (for ATCC 13883) and A2 (for 10.85). Organ burden after 24, 48 or 72h is represented in panels B1 to D1 for ATCC 13883 and B2 to D2 for 10.85. Violet diamond, ATCC 13883 or 10.85 wild type strains. Pink circle, ΔOmpK35OmpK36GD mutant. For survival studies the results were analysed using Long-rank (Mantel-Cox) test and Gehan-Breslow-Wilcoxon test. To compare bacterial load in organs during lung infection, results were compared using Mann-Whitney unpaired t tests. The analyses were performed using Prism7 (GraphPad Software). The differences between the wild type and the porin mutants were not statistically significant (P < 0.05) in any case.