pgen.1008508.g006.tif (442.74 kB)

Loss of mdt-15 renders worms sensitive to cadmium and zinc.

figure

posted on 2019-12-09, 18:31 authored by Naomi Shomer, Alexandre Zacharie Kadhim, Jennifer Margaret Grants, Xuanjin Cheng, Deema Alhusari, Forum Bhanshali, Amy Fong-Yuk Poon, Michelle Ying Ya Lee, Anik Muhuri, Jung In Park, James Shih, Dongyeop Lee, Seung-Jae V. Lee, Francis Christopher Lynn, Stefan Taubert

[A] The graph shows the average number of eggs laid over a 24-hour period by WT worms and mdt-15(tm2182) mutants grown on 0μM and 100μM zinc (Zn). Each dot represents progeny from an individual worm (WT 0μM Zn: n = 59; WT 100μM Zn: n = 56; mdt-15(tm2182) 0μM Zn; n = 57; mdt-15(tm2182) Zn: n = 51; from 3 independent repeats). Error bars: standard deviation; statistical analysis: **** p < 0.0001, Two-way ANOVA, multiple comparisons; Tukey correction. [B] The graph shows the average number of eggs laid over a 24-hour period by WT worms and mdt-15(tm2182) mutants grown on 0μM and 2.5μM cadmium (Cd). Each dot represents progeny from an individual worm (WT 0μM Cd: n = 25; WT 2.5μM Cd: n = 23; mdt-15(tm2182) 0μM Cd: n = 26; mdt-15(tm2182) on 2.5μM Cd: n = 27; from 3 independent repeats). Error bars: standard deviation; statistical analysis: **** p < 0.0001, * p < 0.05, Two-way ANOVA, multiple comparisons; Tukey correction. [C] The graph shows the average number of eggs laid over a 24-hour period by WT worms and hizr-1(am286) mutants grown on 0μM and 100μM zinc (Zn). Each dot represents progeny from an individual worm (all groups n = 46; from 2 independent repeats). Error bars: standard deviation; statistical analysis: *** p < 0.001, **** p < 0.0001, Two-way ANOVA, multiple comparisons; Tukey correction. [D] The graph shows the average number of eggs laid over a 24-hour period by WT worms and hizr-1(am286) mutants grown on 0μM and 2.5μM cadmium (Cd). Each dot represents progeny from an individual worm (all groups n = 30; from 2 independent repeats). Error bars: standard deviation; statistical analysis: * p < 0.05, Two-way ANOVA, multiple comparisons; Tukey correction. [E] The graph shows the average number of eggs laid over a 24-hour period by WT worms and mdt-15(tm2182) mutants fed control or hizr-1 RNAi and grown on 0μM and 10μM zinc (Zn). Each dot represents progeny from an individual worm (all groups n = 34; from 2 independent repeats). Error bars: standard deviation; statistical analysis: **** p < 0.0001, Two-way ANOVA, multiple comparisons; Tukey correction.