Knockdown of RpATG6 results in abnormal distribution of the yolk organelles and less endocytic vesicles in the oocytes.
Left panel: Representative images of cross-sections of early vitellogenic oocytes and 24h-eggs from control and silenced females were observed in the light microscope. The images show an accumulation of larger yolk organelles in the core cytoplasm (*) and an irregular distribution of these organelles in the periphery of dsRpATG6 oocytes and eggs (white arrowheads). Bars: 200 μm. Right panel: Representative images of cross-sections of cortex and plasma membrane of vitellogenic oocytes from control and silenced females observed under the TEM. Silenced oocytes do not show projected microvilli or endocytic vesicles as seen in the control oocytes. Black arrowheads: endocytic vesicles. Bars: 0.5 μm.