Kinetics of pro-apoptotic caspase activation induced by Bi-L-RhamBet.

A549 cells were treated with 1 μM of Staurosporine (STS), 10 μM of x-hederin (Hed) and 5, 10, and 20 μM of Bi-L-RhamBet. The activity of caspase 8 (A), caspase 9 (B) and caspase 3/7 (C) was measured after 2, 4, 6, 8, and 10 h. *Values are significantly different from untreated (control) cells (n = 3); Kruskal-Wallis One Way test followed by post-hoc Student-Newman-Keuls’ test. This analysis is representative of three independent experiments.