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Inward movement of signaling activities is not due to cell movement.

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posted on 2019-10-15, 17:39 authored by Sapna Chhabra, Lizhong Liu, Ryan Goh, Xiangyu Kong, Aryeh Warmflash

(A) Snapshot from time-lapse imaging at 47 h post BMP treatment in the bright field channel. Green and red dots represent initial and final positions, respectively, of labeled cells that were correctly tracked throughout imaging. (B) Histogram of displacement of tracked cells. n = 84 cells. (C) Image of colony (shown in A) immunostained for CDX2, BRA post live cell imaging. Quantification represents average nuclear intensities of indicated markers normalized to DAPI as a function of radial position. Error bars represent standard error of the mean. N = 6. The lines indicate the inner and outer positions of a region of high BRA as a rough guide to the different fate territories (D) Radial displacement of cells as a function of their starting position. Radial displacement = distance of the cell from the center at end of imaging − distance of the cell from the center at the start of imaging. The vertical dotted lines are in the same positions as in (C). The horizontal dashed line separates the cells moving toward the edge from cells moving toward the center. Underlying data can be found in S4 Data. BMP, Bone Morphogenic Protein; BRA, BRACHYURY.

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