Inhibitory effect of Eif2s3y on oogenesis.

(A) Expression level of Y-linked genes in XY oocytes at agg-7. Black bars indicate SD. (B) Expression dynamics of Eif2s3y. The graph shows TPM and SD of Eif2s3y in XX and XY cells. (C) Oocyte differentiation from Eif2s3y-expressing XX and XO ESCs. Images are rOvaries at agg-21. The expression level of Eif2s3y is shown as high, middle or low, based on S6B Fig. Scale bars, 200 μm. (D) The number of oocytes formed in culture. Each dot indicates the number of oocytes formed in one rOvary. The numbers in the graph indicate the average number of oocytes formed in each genotype. P values were calculated by Steel Dwass test. ***P<0.001, **P<0.01, *P<0.05; NS, not significant. (E) Expression dynamics of Eif2s3x. The graph shows TPM and SD of Eif2s3y in XX and XY cells. (F) Total amount of Eif2s3x and Eif2s3y transcripts. (G) Oocyte differentiation from Eif2s3x-expressing XX ESCs. Images are rOvaries at agg-21. The expression level of Eif2s3x is shown as high or middle, based on S6B Fig. Scale bars, 200 μm. (H) The number of oocytes formed in culture. Each dot indicates the number of oocytes formed in one rOvary. The numbers in the graph indicate the average number of oocytes formed in each genotype. P values were calculated by Steel Dwass test. ***P<0.001, **P<0.01, *P<0.05; NS, not significant.