Inhibition of growth and IC₅₀ determination of glucose uptake by isolated P. falciparum parasites.

A) IC₅₀s for inhibition of growth of P. falciparum strain 3D7 intraerythrocytic forms by each compound were determined via a growth inhibition assay as described in Materials and Methods. Data are expressed as means ± SEMs of three independent experiments performed in duplicate. B) Uptake of 2-DG by isolated P. falciparum trophozoites at increasing concentrations of WU-1. Distribution ratios (i.e., the ratio of intracellular concentration of radiolabel relative to the extracellular concentration) were calculated as described previously [23, 50]. IC₅₀s were calculated using nonlinear regression analysis (GraphPad Prism 6.0). Uptake data are expressed as means ± SEMs of three independent experiments performed in triplicate.