IC50 determination of hexose uptake by HEK293 cells overexpressing representative human class II and III orthologues.
A) Uptake of 2-DG by HEK293 cells overexpressing either PfHT or class III GLUT transporter, hGLUT8. B) Uptake of [3H]-D-fructose (Frc) in HEK293 cells overexpressing hGLUT8, or PfHT or class II transporter, GLUT5. Uptake data are expressed as means ± SEMs of three independent experiments performed in triplicate. IC50s were calculated using nonlinear regression analysis (GraphPad Prism 6.0) and are tabulated in Table 2.