pone.0216457.g005.tif (178.74 kB)
IC50 determination of hexose uptake by HEK293 cells overexpressing representative human class II and III orthologues.
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posted on 2019-05-09, 17:44 authored by Monique R. Heitmeier, Richard C. Hresko, Rachel L. Edwards, Michael J. Prinsen, Ma Xenia G. Ilagan, Audrey R. Odom John, Paul W. HruzA) Uptake of 2-DG by HEK293 cells overexpressing either PfHT or class III GLUT transporter, hGLUT8. B) Uptake of [3H]-D-fructose (Frc) in HEK293 cells overexpressing hGLUT8, or PfHT or class II transporter, GLUT5. Uptake data are expressed as means ± SEMs of three independent experiments performed in triplicate. IC50s were calculated using nonlinear regression analysis (GraphPad Prism 6.0) and are tabulated in Table 2.
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zero-trans D-fructose uptakeWUendofacial ligand cytochalasin BMaybridge HitFinder chemical libraryfacilitative hexose transporters10 μ MantagonistN-terminal FLAG tagPfHT over-expressing cell linesPlasmodium falciparum hexose transporter PfHTGLUTECATB-BMPAFLAG-mediated protein engineeringglucose permeation pathway2- DG uptakeglucose transport inhibitorsIC 50throughput screening platformIIIII
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