IC₅₀ determination of glucose uptake by HEK293 cells overexpressing human class I orthologues.

2-DG uptake in response to increasing WU-1 (A) or WU-2 (B) concentrations in HEK293 cells expressing the indicated GLUT protein. IC₅₀s were calculated using nonlinear regression analysis using GraphPad Prism 6.0 and are tabulated in Table 1. Uptake data are expressed as means ± SEMs of three independent experiments performed in triplicate.