IC50 determination of glucose uptake by HEK293 cells overexpressing human class I orthologues.

2-DG uptake in response to increasing WU-1 (A) or WU-2 (B) concentrations in HEK293 cells expressing the indicated GLUT protein. IC50s were calculated using nonlinear regression analysis using GraphPad Prism 6.0 and are tabulated in Table 1. Uptake data are expressed as means ± SEMs of three independent experiments performed in triplicate.