IBA-1 and GFAP staining for reactive glial cells in the medial corpus callosum of mice exposed to filtered air or nanoparticulate matter (nPM).

(A) IBA-1 positive cell counts in each experimental group. The nPM group showed significantly greater microglial cell count (n = 8) compared to the filtered air group (n = 8, p = 0.047). * signifies p < 0.05. (B) Below; low magnification representation of region analyzed. Above; filtered air and nPM exposed mice stained for IBA-1 in the corpus callosum (40x). The upper left hand corner is a high magnification representation of a single cell. (C) IBA-1 cell body to dendritic process ratio in each experimental group. The nPM cohort had a significantly increased ratio (n = 8) compared to the filter group (n = 8, p < 0.001). ** signifies p < 0.001. (D) GFAP positive cell counts in each experimental group. There was no significant difference in astrocyte cell count between groups (p = 0.983) (E) Below; low magnification representation of region analyzed. Above; filtered air and nPM exposed mice stained for GFAP in the corpus callosum (40x). The upper left hand corner is a high magnification representation of a single cell. Error bars represent standard deviation. Scale bars are presented on the lower right corner of the images.