Horizontal gene transfer event in PB-2.

Nespolo, Roberto F.; Villarroel, Carlos A.; Oporto, Christian I.; Tapia, Sebastián M.; Vega-Macaya, Franco; Urbina, Kamila; De Chiara, Matteo; Mozzachiodi, Simone; Mikhalev, Ekaterina; Thompson, Dawn; Larrondo, Luis F.; Saenz-Agudelo, Pablo; Liti, Gianni; Cubillos, Francisco A.

doi:10.1371/journal.pgen.1008777.s005

pgen.1008777.s005.pdf (345.32 kB)

Horizontal gene transfer event in PB-2.

journal contribution

posted on 2020-05-01, 17:32 authored by Roberto F. Nespolo, Carlos A. Villarroel, Christian I. Oporto, Sebastián M. Tapia, Franco Vega-Macaya, Kamila Urbina, Matteo De Chiara, Simone Mozzachiodi, Ekaterina Mikhalev, Dawn Thompson, Luis F. Larrondo, Pablo Saenz-Agudelo, Gianni Liti, Francisco A. Cubillos

(A) Principal component analysis using only non-admixed 83 isolates from all populations shows partial concordance with the phylogenetic tree. The first component clearly separates the Chinese/North American branch from the South American lineages. Second component identifies the PB-3 as the most separated lineage, suggesting a lower level of outbreeding, while a partial overlap can be identified between the other lineages. The middle positioning of PB-1 using the third component mirrors the shape of the un-rooted phylogenetic tree based on the sequence divergence (Fig 2). (B) Nine ORFs, within the denominate Region A, have been identified on a single contig in 9 isolates. Around 6 kb of the flanking regions of these ORFs correspond to the chromosome I subtelomere, while the region where the ORFs are located do not show any similarities with known regions. (C) In the aminoacidic sequence of the nine ORFs, several domains can be identified of inferred by homologies.

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