pone.0225658.g003.tif (7.22 MB)
Heat map view of the final 96.96 Dynamic Array run with individual assays on the x axis and individual samples on y axis.
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posted on 2019-12-03, 18:44 authored by Dominika Borowska, Richard Kuo, Richard A. Bailey, Kellie A. Watson, Pete Kaiser, Lonneke Vervelde, Mark P. StevensThe RNA extracted from tissue samples from broilers raised in two different biosecurity environments (sibling-test and pedigree farms); samples from five tissues from four birds on each of the two farms were used; pooled preamplified cDNA was used for standard curve dilutions.
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multiplexed qPCR 96.96 Fluidigm Dynamic Array6 nanolitre reactionsbroiler chickensPCR-based platformmicrofluidic system96 transcriptstranscript levelstranscriptome dataamplicon sequencingchicken macrophagesRNA-seq analysispreamplification stepsConventional qPCR analysisGut-associated lymphoid tissuesblood cellstranscriptional response89 genesimmune-related genesbiosecurity levelsmultiplexed qPCR-based platformscreen vaccine responseschicken immune-related genesmultiplex qPCR array96 samplesanalysehigh-throughput assaysblood leukocytescurve analysispathogen-specific probesdendritic cells
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