HT1080 cellular uptake of 57Co-Cbl in the presence of either FCS or HS with or without heat treatment.
A, FCS was heated at either 95°C for 10 min or 100°C for 20 min before 57Co-Cbl addition, then incubated with HT1080 cells for 48 h and compared to standard FCS culture conditions. B, Using the same 95°C for 10 min FCS heating conditions used in “A”, a time-course study with samples taken at 2 h, 4 h, 8 h, 12 h, 24 h, and 48 h was undertaken. C, For the purpose of comparison, a time-course study with heated HS (95°C for 10 min) was also conducted in parallel. Data are mean values + SE (n = 3).