pgen.1008435.g005.tif (518.77 kB)
Growth of ΔyddB and ΔpqqL mutant strains is identical to wildtype in iron limited LB media.
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posted on 2019-10-15, 17:25 authored by Rhys Grinter, Pok Man Leung, Lakshmi C. Wijeyewickrema, Dene Littler, Simone Beckham, Robert N. Pike, Daniel Walker, Chris Greening, Trevor Lithgow(A) E. coli BW25113 wildtype, ΔyddB and ΔpqqL strains were grown in LB media +/- 80 μM 2,2’bipyridine. Growth of ΔyddB and ΔpqqL strains was identical to wildtype under both conditions; all strains exhibited slower growth with 80 μM 2,2’bipyridine due to iron limitation of the media. (B) E. coli BW25113 ΔTBDT and ΔTBDT/ΔyddB were grown as strains in panel A. Growth of ΔTBDT/ΔyddB was identical to the ΔTBDT strain under both conditions; both strains grew slower than wildtype due to poor iron uptake ability, with very slow growth observed with 80 μM 2,2’bipyridine.
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gene clustersGram-negative plant pathogensferredoxin uptake systemM 16 protease familyferredoxin processing protease FusCStructural analysisFusC shareFus homologues YddBgene cluster encoding proteinsuptake machineriesmembrane protein YddB sharesperiplasmic localisationplant hostsgenus Pectobacteriumprotein ferredoxinextracellular substrate binding siteEscherichia coliGram-negative bacteria BacteriaGram-negative bacteriaProtease-associated import systemsiron-dependent expressiontransport ferredoxinimport systemsmembrane ferredoxin transporter FusAPqqL
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