Growth of ΔyddB and ΔpqqL mutant strains is identical to wildtype in iron limited LB media.

(A) E. coli BW25113 wildtype, ΔyddB and ΔpqqL strains were grown in LB media +/- 80 μM 2,2’bipyridine. Growth of ΔyddB and ΔpqqL strains was identical to wildtype under both conditions; all strains exhibited slower growth with 80 μM 2,2’bipyridine due to iron limitation of the media. (B) E. coli BW25113 ΔTBDT and ΔTBDT/ΔyddB were grown as strains in panel A. Growth of ΔTBDT/ΔyddB was identical to the ΔTBDT strain under both conditions; both strains grew slower than wildtype due to poor iron uptake ability, with very slow growth observed with 80 μM 2,2’bipyridine.