Genes that function in stress response, morphogenesis, and membrane trafficking influence copper resistance.

2019-01-11T18:38:04Z (GMT) by Lois M. Douglas James B. Konopka
<p>(A) <i>C</i>. <i>albicans</i> strains were replica-plated onto minimal synthetic media containing copper or another indicated metal, and then the extent of growth was recorded and summarized in a heat map. The mutant strains clustered into three major groups. The <i>sur7Δ</i> mutant clustered with other mutants known to be defective in trafficking and morphogenesis, a separate cluster included the <i>HOG1</i> pathway mutants that are sensitive to stress, and the known copper mutants <i>crp1Δ</i> and cup2<i>Δ</i> clustered. The results represent a compilation of three experiments for each metal performed on different days. (B) The sensitivity of <i>sur7Δ</i> to cadmium (Cd), chromium, (Cr), and cesium (Cs) was further tested by incubating cells in different concentrations of these metals in synthetic medium a 96-well plate, since replica-plating indicated some degree of growth inhibition in response to these metals. Results indicated that <i>sur7Δ</i> cells only showed strong sensitivity to copper. The graph represents the averages of two to three independent experiments performed on different days.</p>