Gata4 is required in Hh-receiving cells for OFT development.
(A-K’) Histology of Gata4 transgenic mouse embryonic heart at E14.5. The lower panels (A’-C’) and upper panels (D-K) showed that the opening of the AO connected to the right ventricle in the Gata4 haploinsufficency, while in control embryos the AO opened to the left ventricle. LV, left ventricle; RV, right ventricle; ao, aorta artery; pt, pulmonary trunk. Magnification: 40X (L-M) Blue ink stained embryo heart at E14.5. LV, left ventricle; RV, right ventricle; ao, aorta artery, pt, pulmonary trunk. Red arrow indicates the ink injected direction.