Gastrointestinal transit of luminescent <i>L</i>. <i>reuteri</i> strains.

<p>Localization of 6475-CBRluc-mCherry, R2LC-CBRluc and wildtype strains was evaluated <i>in vivo</i> and <i>ex vivo</i> using IVIS at 0, 60, 120 and 180 min after administration of a single dose of the bacteria (1x10<sup>10</sup> CFU/mouse) in 3 separate experiments. 6475-CBRluc-mCherry (n = 10), R2LC-CBRluc (n = 3), 6475-wild type (n = 3) and R2LC-wild type (n = 1) for luminescence and 6475-CBRluc-mCherry (n = 5) and 6475-wild type (n = 2) for fluorescence imaging. 6475-wildtype (<b>a, b</b>); 6475-CBRluc-mCherry 0 min (<b>c, d</b>); 60 min (<b>e, f</b>); 120 min (<b>g, h</b>); and 180 min post gavage (<b>i, j</b>); R2LC-wildtype (<b>k, l</b>); R2LC-CBRluc 60 min (<b>m, n</b>) and 180 min post gavage (<b>o, p</b>).</p>