GBA2 silencing reduces GBA2 activity in CuFi-1 cells.
CuFi-1 cells were transfected with GBA2 siRNA or scrambled oligonucleotides as indicated in figure 5. Eighteen or 42 hours after transfection, the cells were scraped and treated as indicated in figure 2. Total β-glucosidase (B) and GBA2 (A) activities were measured as reported in the Methods section. The data reported are the mean ± standard error of the mean of 2 independent experiments in triplicate. Comparisons between groups were made by using Student’s t tests.