Functional consequences of piceatannol binding to glyceraldehyde-3-phosphate dehydrogenase - Fig 3

(A) Thermal effects of the titration 0.5 mM piceatannol solution (in the syringe) into 5 μM GAPDH protein solution (in the cell) (■) and corresponded to them thermal effects of dilution of the piceatannol in pure water (○). (B) Thermal effect of the interaction between protein GAPDH and piceatannol corrected with the piceatannol dilution effects and calculated per one mole of the ligand. Solid line is approximation of ITC date of GAPDH by piceatannol using the model of one set of binding sites.