Formation of XX, XO and XY oocytes in culture.
(A) Schematic illustration of the experimental design. PGCLCs derived from XX, XO and XY ESCs were reaggregated with E12.5 female (XX) gonadal somatic cells. (B) Oocyte differentiation from XX, XO and XY ESCs in culture. Images are rOvaries at the days indicated. Blimp1-mVenus (BV) is a marker of PGC(LC)s and stella-CFP (SC) is a marker of PGC(LC)s and oocytes. BF, bright field. Scale bars, 200 μm. (C) The number of oocytes formed in culture. Each dot indicates the number of oocytes formed in one rOvary. The numbers in the graph indicate the average number of oocytes/rOvary formed in each genotype. XpO and XmO indicate cells having a paternal and maternal X chromosome, respectively. (D) Time-course of the number of PGCLCs/oocytes. The average number of oocytes at the days indicated and SD are shown. The data was compiled from at least three independent experiments. P values were calculated by Steel Dwass test. ***P<0.001, **P<0.01, *P<0.05; NS, not significant.