Fluorescence of PI (A), Hoechst (B) and FDA (C) stained cercariae over time.

Each sample contains 100 ± 4 cercariae. Dead cercariae were heat-killed at 60°C for 5 minutes, and mixed samples contain 50% alive and 50% dead cercariae. Data points and error bars show the mean and SEM of nine wells (experiments run in triplicate and repeated three times).