Fluorescence correlation spectroscopy data for GFPcomp-labeled species (green) and mCherry-labeled species (red).

The experimental autocorrelation data, fitted curves (in black), and residuals, are shown for an individual representative HEK-293T cell. A. Control cell transiently expressing MBD-GFPcomp and mCherry and infected with the WSN-wt virus; single-component translational diffusion model. Arrow points to the inflection point of the autocorrelation curve. B. Cell transfected with GFP1-10 and NP-mCherry and infected with the WSN-PB2-GFP11 virus. Two-component translational diffusion model.