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Flow cytometric analysis confirmed that both bNK2A peptides induced damage to Histophilus somni membranes.

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posted on 2019-06-19, 17:37 authored by Rohana P. Dassanayake, Shollie M. Falkenberg, Eric M. Nicholson, Robert E. Briggs, Fred M. Tatum, Vijay K. Sharma, Timothy A. Reinhardt

Live and dead H. somni in PBS control (A, PBS), heat-killed at 56°C for 10 min (B), and treated with 20 μM bNK2A-C10C20 (C), or 20 μM bNK2A-S10S20 (D) at 37°C for 30 min were stained with LIVE/DEAD BacLight bacterial viability dyes. X-axis indicates live bacteria (Syto 9) and Y-axis indicates dead bacteria (propidium iodide). Results of one representative experiment out of three are shown.

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