Flow cytometric analysis confirmed that both bNK2A peptides induced damage to Histophilus somni membranes.

Live and dead H. somni in PBS control (A, PBS), heat-killed at 56°C for 10 min (B), and treated with 20 μM bNK2A-C10C20 (C), or 20 μM bNK2A-S10S20 (D) at 37°C for 30 min were stained with LIVE/DEAD BacLight bacterial viability dyes. X-axis indicates live bacteria (Syto 9) and Y-axis indicates dead bacteria (propidium iodide). Results of one representative experiment out of three are shown.