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FN promotes HBV expression by activating HBV EI and EII activities.

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posted on 29.03.2016 by Sheng Ren, Jun Wang, Tie-Long Chen, Hao-Yu Li, Yu-Shun Wan, Nan-Fang Peng, Xi-En Gui, Ying Zhu

(A) Huh7 cells were transfected with HBV EI or EII along with pFN and then cell culture medium was refreshed with serum-free medium 6 h post-transfection. Luciferase activity was measured 24 h after serum starvation. Huh7 cells were transfected with wild-type EI or mutant EI HNF-4α-Mut (B), wild-type EII or mutant EII HNF-4α-Mut (C) along with shFN or shNC, and then cell culture medium was refreshed with serum-free medium 6 h post-transfection. Luciferase activity was measured 24 h after serum starvation. (D) Huh7 cells were transfected with shFN or shNC. Cells were lysed 48 h post-transfection and HNF-4α levels were determined by qRT-PCR (upper panel) and western blot (lower panel). (E) Huh7 cells were transfected with shFN or shNC along with pHBV and then ChIP analysis was performed to assess HNF-4α binding capability to HBV enhancers 48 h post-transfection. All experiments were repeated at least three times with similar results. Data represent means ± SD, n = 3 (*p<0.05).

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