Expression profile of mean RPKM values of Nitrate Assimilation components in Fruit and Leaf tissue.

A. RPKM and RT-qPCR values for Nitrate Reductase. B. Low affinity nitrate transporters NRT1-2-11 and NRT1-4-6 C & D. RT-qPCR expression values for the highest expressed low and high affinity nitrate transporters. E. High affinity nitrate transporters NRT2-1.2, NRT2-1.1. RT-qPCR results (A, C and E) are averages of the number of replicates (N) and bars indicate the standard deviation between replicates. Table of annotations and accession numbers from Sol Genomics ITAG3.2. RT-qPCR relative values for tissue type and treatment, trend with the corresponding RPKM values generated from the HTS analysis. Error bars denote the standard deviation of RT-qPCR expression values between 3 biological replicates and at least 3 technical replicates. Error bars denote the standard error of sequenced expression values between 3 biological replicates.