Figure_1.tif (361.14 kB)
Expression of the sialidase and selected hyaluronidase genes in the wild-type, mutant and complemented strains.
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posted on 2013-09-04, 01:29 authored by Thomas J. Hiscox, Paul F. Harrison, Anjana Chakravorty, Jocelyn M. Choo, Kaori Ohtani, Tohru Shimizu, Jackie K. Cheung, Julian I. RoodRNA was isolated from cells grown in TPYG broth for 4 h, which corresponds to exponential growth phase. The relative expression of the nanI, nanJ, nagJ and nagL (A) and nagK (B) genes was determined by QRT-PCR. Expression levels are relative to the expression of the housekeeping gene rpoA and are the average of three independent biological replicates (± SEM). The asterisk (*) denotes a statistically significant difference (p≤0.05) as calculated using the student’s t-test.