Experimental design.

All treatment groups (rows) were transferred to environmental chambers as eggs within 15 hours of being laid, and all developed into adults by the end of the experiment. “wMel” denotes the wMel-infected A. aegypti controls, and “Cairns” denotes the wildtype (Wolbachia-free) A. aegypti controls. For each of the other treatment groups, the letter represents the stage of heat onset, with “E” indicating embryogenesis, “I” indicating immature larvae (1st/2nd instars), and “M” indicating more mature larvae (3rd/4th instars). The number represents the number of days the group remained in the high temperature treatment before returning to control temperatures (three days, five days, or seven days). Moving across each row, the cells track the days for each treatment group, with blue cells representing days spent in the control chamber and red cells representing days in the high temperatures chamber. Inverted triangles represent adult collection time points at 0–2 days and 4–7 days after emergence.