Exercise induces p21 expression in both control and TPA-promoted skin epidermis of mice.
SENCAR mice were performed treadmill exercise at 20 m/min, 60 min/day and 5 days/week for 10 weeks. The dorsal skin of each mouse was then treated with vehicle control or TPA at 3.2 nmol and analyzed for protein expression two hours after TPA treatment. (A) Representative confocal microscopy images of p21 fluorescence intensity (top) and quantification of IHC images (bottom). (B) Representative Western blots of p21 protein (top) and quantification of Western blot by densitometry (bottom). The data were normalized to the level of expression in one of the control and present as mean ± SD of three to four independent experiments. *, p < 0.05 versus sedentary control with vehicle or TPA treatment, respectively. Sed, sedentary control; Ex, exercised; Ace, acetone.