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Epsilon toxin does not bind to the microvasculature of other peripheral organs.

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posted on 2019-11-08, 18:34 authored by Jennifer R. Linden, Claudia Flores, Eric F. Schmidt, Francisco A. Uzal, Adam O. Michel, Marissa Valenzuela, Sebastian Dobrow, Timothy Vartanian

Wild type mice expressing Mal+/+ or mice deficient in Mal-/- were intravenously injected ETX-594 for ten minutes then perfused with PBS to remove unbound toxin. ETX binding to microvasculature was evaluated in tissue cyrosections. FITC-BSL1 was used to visualize microvasculature. ETX bound to the epithelial cells of renal tubules of Mal+/+ mice (white arrows) but not the glomerular capillaries (asterisks). In Mal-/- mice, ETX, can be seen accumulating in unidentified renal structures (white arrow heads). ETX is also observed binding to the microvasculature of the intestines in Mal+/+ mice (white arrows), but not Mal-/- mice. ETX binding was not observed in the spleen, lung, liver or heart of Mal+/+ or Mal-/- animals.

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