Effects of procyanidins, EC and C3G on the plasma glucose level in an oral glucose tolerance test.

ICR mice were dosed orally with 0.001 to 1000 μg/kg body weight of polyphenols or water (5 mL/kg body weight). Sixty minutes after the administration, the mice were orally given glucose solution (1 g/kg body weight) followed by plasma glucose measurements at 0, 15, 30, 60 and 120 min. (A) Results of oral glucose tolerance tests were shown after the treatment with 0.001 (■), 0.1 (▲) or 10 (●) μg/kg body weight of PA 4–2 or water [5 mL/kg body weight; (●)]. Values are means ± SE (n = 6). Values of area under the curve (AUC) calculated from the treatment with 0.001 to 1000 μg/kg body weight of polyphenols are shown in the panels B to H. (B) AUC from the treatment with 0.1μg/kg body weight of polyphenols. (C-H) AUC from the treatment with each polyphenol at different concentrations. Values are means ± SE (n = 6). *Significantly different from the corresponding control group (p <0.05; Dunnett’s test in the panels A and C to H). Different superscripted letters indicate significant differences between the groups (p <0.05; Tukey-Kramer multiple comparison test in the panel B).