Effects of claudin-4 knockout on the localization of other claudins.

(A) Immunofluorescence analysis of claudins in the co-culture of wild-type MDCK II cells and claudin-4 knockout clone 3 (sKO3). No apparent difference was observed between the localization of claudin-1, -2, -3 and -7 in the wild-type cells and claudin-4 knockout clone. Scale bar = 10 μm. (B) Quantification analysis of the signal intensity of claudins at TJs in wild-type cells and claudin-4 knockout clone. The signal intensity of claudins at TJs in wild-type cells and claudin-4 knockout clone 3 was measured as described in Materials and Methods, and the relative signal intensity of each claudin was calculated as the ratio of the signal intensity in wild-type cells (CTL) and claudin-4 knockout clone 3 (sKO3) to the signal intensity in wild-type cells. The signal intensity of claudins at TJs was not significantly different between wild-type cells and claudin-4 knockout clone. N = 4 for each experiment.