Effects of acclimatization on the nociceptive response threshold evoked by calibrated forceps.

(A) The threshold distribution of acclimatized (open circles) and unacclimatized (filled circles) mice (n = 6, respectively) were plotted. (B) The mean values of the threshold were replotted. The distribution was not significantly different between the two groups (t = 0.6747, df = 10, p = 0.5152, unpaired t test). (C) The SEM values of the thresholds were replotted. The value was significantly lower in acclimatized mice (t = 2.700, df = 10, p = 0.0223, unpaired t test). Bars indicate mean ± SEM. A, acclimatized group; UA, unacclimatized group